LEICA 顯微鏡系統
LEICA 雷射掃描共軛焦顯微鏡
.LEICA TCS SP8 光譜式共軛焦顯微鏡系統
.LEICA TCS SP8 共軛焦技術與應用
.LEICA HyD 共軛焦光譜偵測器
.LEICA TCS SP8 DLS 共軛焦-光層切片掃描螢光影像系統
.ANDOR DSD Confocal system
.Yokogawa Spinning disk confocal
LEICA 超解析顯微影像系統
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CherryTemp超高速瞬間溫控檯
Tomocube 無螢光標記活細胞全息影像系統
ANDOR 科研相機及影像系統
LifeCanvas 組織澄清透明化處理系統
顯微鏡自動化與相關設備
影像控制分析軟體
顯微操作注射與組織切割系統
半導體及工業用顯微鏡
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首頁 > LEICA 雷射掃描共軛焦顯微鏡 > LEICA TCS SP8 光譜式共軛焦顯微鏡系統
LEICA TCS SP8 MP

Leica TCS SP8 MP 多光子影像系統, 是架構在 TCS SP8 , 可升級採用 Ti:Sapphire 飛秒雷射 ( Femto second Pico second, OPO IR laser  ), 因為減少光的吸收及激發光的散射, 所以, 可減少光漂白及光毒害, 可做厚樣品的深層影像擷取., 例如活體的細胞, 器官, 胞器, 組織.. 等樣品.  新一代雷射的發展, 如 OPO IR 雷射, 可讓使用者擷取多色螢光的多光子影像 ( Multicolor MP imaging )

獨家多光子影像專屬物鏡及 HyD NDD 偵測器, 讓使用者看得更深, 更細.



Principle of Multiphoton Microscopy

The probability of excitation in one-photon excitation (left) linearly depends on the amount of photons from the light source. In two-photon excitation (right) it is proportional to the square of the intensity of the light source and limited to the vicinity of the focal plane.




降低雷射激發光的散射, 所以更能深入穿透樣品, 適於厚樣品的應用.

  • 激發光僅限於焦平面的極小點, 不是整個大面積的區域, 減低雜訊. 避免快速螢光漂白.
  • 無須使用 Pinhole
  • 減低光毒害, 適合活細胞影像擷取.
  • 極適合應用於 uncaging, photoactivation, Photobleaching

    一般多光子影像的應用領域

    Neuroscience

    • Calcium Dynamics in Brain Slices

    • Neuronal plasticity

    • Synaptic physiology

    • Spine structure-function relationship

    • Dendritic signalling

    Immunology

    • Lymphocyte trafficking

    • Immune system dynamics

    Development

    Pathological conditions
    (Development, progression and potential treatment)

    • Tumors (angiogenesis / metastasis)

    • Alzheimer disease

    Diverse specimens:

    • Lymphatic organs

    • Kidney

    • Heart

    • Skin

    • Brain




    有效觀察深度 約 300 – 700 µm

  • 飛秒雷射光源 :
    1). picosecond lasers
    2). femtosecond lasers
    3). femtosecond lasers with precompensation
    4). OPO (optical parametric oscillator)

     

    Superior Optics and Detection for Brighter, High-Contrast Multiphoton Images

    Leica’s broadband anti-reflective coatings for scan optics and objectives provide highest transmission in the visible and for optimal excitation. Leica IRAPO high-NA objectives are color corrected in the infrared up to 1300 nm to minimize the axial shift

    Super-sensitive HyD non-descanned detectors efficiently collect the emitted photons resulting in brighter images from deeper tissue sections and minimizing photodamage to the specimen










    HC PL IRAPO

    • high transmission in visible and infrared up to 1300 nm
    • color correction in infrared
      for 20x objective extended to the visible
    • excellent field flatness (PL)

    HCX IRAPO L

    • high transmission in visible and infrared up to 1000 nm
    • color correction in visible reaching into infrared
    • very large free working distance (L)










    More Data in Less Time – Largest Field of View at Highest Speed

    At 22 mm the exceptionally large field of view of the Leica TCS SP8 allows scanning of large areas of tissue at once minimizing the number of scans.

    Even at the high speed possible with the 12 kHz scanner, no compromise in resolution, sensitivity and contrast is required thanks to high-numerical aperture objectives with long free working distance and the super-sensitive Leica HyD non-descanned detectors.




    Deep Tissue Imaging

    Ureteric bud from an embryonic day 16 kidney from a HoxB7 EGFP mouse.



    Simultanous excitation with OPO and Ti:Sa

    Mouse mammary gland (left) and spleen (right). Blood vessels labelled with 70kD-Texas Red excited with OPO at 1150 nm (red). Simultaneous excitation at 800 nm results in second harmonic generation  (SHG) signal of type I collagen (purple) and autofluorescence of single cells (green).  Courtesy of Evelyne Beerling, Jacco van Rheenen, Hubrecht Institute, Utrecht, The Netherlands



     

    相關連結
    LEICA TCS SP8 MP 網站
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