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首頁 > LEICA 雷射掃描共軛焦顯微鏡 > LEICA TCS SP8 光譜式共軛焦顯微鏡系統
LEICA TCS SP8 CARS

近代非線性光學影像技術具有高靈敏度、高解析度、單一性, 低破壞性、能夠光切三維成像、且可以做更深層的成像等優點,因此, 除了多光子影像技術已被廣泛應用於各種生物醫學相關議題外, 同調反史托克拉曼散射 (Coherent anti-Stokes Raman scattering, CARS) 顯微技術為最新崛起的非線性光學影像技術,除了具備其他非線性光學影像方法之優點外,無須染色便具有超解析的化學鑑別力,成像可從細胞分子到微小生物樣本, 使其在生醫研究上具有無限的發展潛力。

LEICA TCS SP8 CARS 實現了極簡易使用的優勢, 讓生醫界用戶, 能專注樣本, 輕鬆取得無須染色的高清晰影像.



For imaging with the Leica TCS SP8 CARS confocal microscope, specimen do not have to be stained. The Coherent anti-Stokes Raman Scattering (CARS) technology involves two laser beams to visualize the vibrational contrast of molecules in specimens. The specimen remains almost unaffected.

The user profits of a wide range of live cell imaging with the combination of visible, IR and UV lasers, second harmonic generation and CARS such as analyzing living cells, tissues and even small whole animals at high speed or at high resolution.

With its flexibility and convenient handling, the Leica TCS SP8 CARS is the ideal device for research institutes and multi-user facilities



















無須螢光染色的自然樣本成像

The integration of the label-free CARS technology into the Leica TCS SP8 confocal platform overcomes the limitation of dye-based imaging methods. Imaging of the characteristic intrinsic vibrational contrast of the pure molecules in the sample does not require any staining. This method is non-toxic, non-destructive and minimally invasive.


單一光學影像平台, 可以使用 VIS, UV, IR, SHG 及 CARS

The Leica TCS SP8 CARS is a platform providing various imaging methods such as CARS, single photon or multiphoton fluorescence or for imaging with UV lasers in simultaneous or sequential mode.

Laser sources, scanner model and imaging mode can be optimally adapted to the application of interest and setup through the system software Leica LAS AF. The ergonomic and intuitive handling ensures that ambitious experiments run smoothly.

在同一系統, 同時兼容高速與高解析成像

Leica TCS SP8 CARS includes the Leica Tandem Scanner. It comprises a conventional scanner for all morphology and classical speed live cell applications and a resonant scanner that provides a wide range of high speed options. This combination of two scanners covers a multitude of applications and makes the Leica TCS SP8 CARS a flexible device fitted for multi-user facilities.

完美的系統整合, 流暢的簡易操作, 最適宜生物醫學用戶.

The compact CARS laser is fully controlled through the system software LAS AF. The tight integration of the laser control into the software makes the sophisticated process of adjusting multiple parameters of the system completely transparent. This solution offers the most convenient way to perform CARS experiments – for experts and novices alike.


CARS 具有下列得使用優勢 :

  • 無須染色便具有化學鑑別力
  • 非線性光學影像技術
  • 可以對樣品進行光譜研究,還可以對其進行功能性成像,提高了分子分析的信息量
  • 高靈敏度
  • 高解析度
  • 低破壞性
  • 非破壞性的三維成像
  • 更深成像深度
  • 具有化學專一性
  • 觀測活細胞內各種胞器或蛋白質
  • 研究具有非中心對稱之結構蛋白(例如膠原蛋白)
  • 不須對樣品進行切片或染色,可即時顯示組織內脂肪微粒分佈,並得到肝臟內脂肪濃度的資訊
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